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Preparation and characterization of PVA/chitosan nanofibers loaded with Dragon's blood or poly helixan as wound dressings

Mahboubi Kancha, M ; Sharif University of Technology | 2024

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2024.132844
  3. Publisher: 2024
  4. Abstract:
  5. Nanofibers have been investigated in regenerative medicine. Dragon's blood (DB)- and poly helixan PF (PHPF) are natural materials used in cosmetics. Herein, we generated DB- and PHPF-loaded polyvinyl alcohol/chitosan (PVA/CS/DB and PVA/CS/PHPF, respectively) nanofibers. PVA/CS/DB and PVA/CS/PHPF nanofibers had an average diameter of 547.5 ± 17.13 and 521 ± 24.67 nm, respectively as assessed by SEM, and a degradation rate of 43.1 and 47.6 % after 14 days, respectively. PVA/CS/DB and PVA/CS/PHPF nanofibers had a hemolysis rate of 0.10 and 0.39 %, respectively, and a water vapor transmission rate of ∼2200 g.m−2.day−1. These nanofibers exhibited favorable antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis in vitro. PVA/CS/DB and PVA/CS/PHPF nanofibers demonstrated a sustained release of 77.91 and 76.55 % over 72 h. PVA/CS/DB and PVA/CS/PHPF nanofibers had a high rate of cytocompatibility and significantly improved the viability of NIH/3T3 cells as compared with free drugs or unloaded nanofibers. Histological inspection via H&E and Verhoeff's staining demonstrated PVA/CS/DB and PVA/CS/PHPF nanofibers enhanced the wound healing and damaged tissue recovery of unsplinted wound models by promoting epithelial layer formation, collagen deposition, and enhancing the presence of fibroblasts. Conclusively, PVA/CS/DB and PVA/CS/PHPF can be introduced as potential wound dressing candidates with favorable properties. © 2024 Elsevier B.V
  6. Keywords:
  7. Chitosan ; Nanofiber ; Wound dressing ; Animals ; Anti-Bacterial Agents ; Bandages ; Cell Survival ; Chitosan ; Hemolysis ; Mice ; Nanofibers ; NIH 3T3 Cells ; Plant Extracts ; Polyvinyl Alcohol ; Wound Healing ; Bacteriology ; Blood ; Cell culture ; Degradation ; Escherichia coli ; Antiinfective agent ; Dragon blood ; Plant extract ; Anti-microbial activity ; Average diameter ; Chitosan nanofibers ; Degradation rate ; Dragon's blood ; Haemolysis ; Natural materials ; Regenerative medicine ; Water vapor transmission rate ; Wound dressings ; Biocompatibility ; Biodegradability ; Biofilm ; Blood clotting ; Cell proliferation ; Cell proliferation assay ; Cell viability ; Cell viability assay ; Cross linking ; Drug release ; Drug synthesis ; Electrospinning ; Fourier transform infrared spectroscopy ; Histology ; Histopathology ; Image analysis ; Immunohistochemistry ; Infrared spectroscopy ; MTT assay ; NIH 3T3 cell line ; Scanning electron microscopy ; Tissue injury ; Animal ; Bandage ; Chemistry ; Drug effect ; Mouse ; Nanofibers
  8. Source: International Journal of Biological Macromolecules ; Volume 272 , 2024 ; 01418130 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0141813024036493