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Design, Fabrication and Optimization of the Drug Delivery System Based on the Features of the Extracellular Matrix with the Aim of Improving the Treatment Process of the Damaged Tissue

Rezaei Demneh, Mohammad Hossein | 2025

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 58742 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo , Amir; Aryanpour, Masoud
  7. Abstract:
  8. Injuries to the peripheral nervous system, parallel muscle tissues, and similar sensitive tissues such as the heart and bone are considered one of the fundamental challenges in regenerative medicine and can result in motor and sensory disorders and, in some cases, permanent disabilities. Despite their relative effectiveness, common treatments such as autologous nerve transplantation have limitations such as lack of donor tissue and the possibility of incomplete healing. Therefore, the development of novel approaches using tissue engineering has gained special importance. The present study aimed to design and fabricate a multifunctional drug-delivery artificial scaffold that simultaneously incorporates three key capabilities: structural guidance of cell growth with the co-directional structure of alginate microfibers, stepwise and sequential drug delivery with a layer-by-layer structure of core-shell microspheres inside alginate microfibers that are located in a chitosan-gelatin hydrogel. And improved electrical conductivity by loading iron oxide nanoparticles. For this purpose, first, parallel and porous alginate microfibers were produced and oriented by jet spraying in various diameters of 50, 100 and 150 μm to create an oriented structure for tissues that require this capability, such as nerve, muscle, vascular and bone tissue. Next, PVA/PCL core-shell microspheres with an average diameter of 15 μm were loaded into the microfibers for sequential drug release, and chitosan gelatin hydrogel was used to increase biocompatibility and cell adhesion. Finally, by adding iron oxide nanoparticles, the scaffold, in addition to improving electrical conductivity, also acquired magnetic stimulation and imaging tracking capabilities. The results of various tests were used to optimize the compositions and structure of this scaffold. By combining three main features, this innovation provides an efficient platform for the regeneration of tissues requiring these capabilities, such as peripheral nerves, muscle, and bone, and offers multifaceted advantages over previous approaches that have often focused on one dimension. Finally, adhesion, cell viability, and cell differentiation tests were used to prove the ability of this artificial tissue for cell growth, proliferation, and differentiation, and the results showed a 1.5-fold improvement compared to the control condition for adhesion viability and favorable differentiation for bone cells
  9. Keywords:
  10. Tissue Engineering ; Targeted Druy Delivery ; Oriented Microstructure ; Drug Delivery Microfibers ; Scaffold Conductivity ; Extracellular Matrix

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