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  1. سنتز نانوذره مکسین برای تثبیت آنزیم و کاربرد آن در حذف آلاینده های نوظهور
سنتز نانوذره مکسین برای تثبیت آنزیم و کاربرد آن در حذف آلاینده های نوظهور
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سنتز نانوذره مکسین برای تثبیت آنزیم و کاربرد آن در حذف آلاینده های نوظهور

حافظی، امیر رضا Hafezi, Amir reza

Synthesis of MXene Nanoparticles for Enzyme Immobilization and their Application in Removing Emerging Contaminants

Hafezi, Amir reza | 2025

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 58226 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheila; Zahra Ghobadi Nejad
  7. Abstract:
  8. One of the major environmental challenges in recent decades has been the presence and accumulation of micropollutants in water resources and natural ecosystems, leading to numerous adverse consequences for the environment and the health of living organisms. Among these compounds, endocrine-disrupting pollutants are of particular importance due to the hormonal disturbances they cause. Bisphenol A (BPA) is one of the most well-known endocrine disruptors, raising significant concerns regarding its environmental and health risks. Traditional methods for removing these pollutants include biological and physicochemical processes, which often lack sufficient efficiency for complete BPA removal. Additionally, advanced oxidation processes, which have been increasingly used, can lead to the formation of toxic and hazardous byproducts. For this reason, enzymatic technology has emerged as an innovative and environmentally friendly approach, offering a promising solution for BPA removal. The enzyme laccase has attracted considerable attention due to its high capability in oxidizing and degrading a wide range of pollutants. Belonging to the oxidoreductase group, laccase can effectively break down BPA. In the present study, laccase was extracted and produced from the white-rot fungus Trametes sp. To enhance the enzyme's stability and reusability, Ti₃C₂ (titanium carbide) nanoparticles from the MXene family were employed as an immobilization support. The laccase enzyme was covalently immobilized onto the functionalized surface of the nanoparticles, significantly reducing enzyme leakage and improving activity recovery. The performance of the immobilized laccase on Ti₃C₂ nanoparticles in BPA removal was evaluated under optimized conditions. Under these conditions, the BPA removal efficiency exceeded 97% within 8 hours. The kinetic parameters, Kₘ and Vₘₐₓ, for the immobilized enzyme on Ti₃C₂ nanoparticles were calculated as 0.142 mM and 0.212 mM/min, respectively, indicating favorable enzymatic performance. Furthermore, the surface structure of the immobilized enzyme was analyzed using scanning electron microscopy (SEM), and its stability and reusability were tested over multiple cycles. The results demonstrated that even after the third cycle, more than 55% of the enzyme's initial pollutant removal capacity was retained
  9. Keywords:
  10. MXene Nanosheets ; Bisphenol A ; Laccas Enzyme ; Biocatalyst ; Emerging Pollutants ; Enzyme Stabilization

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