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Design and Fabrication of Suitable Microchip for Separation of Melittin from Bee Venom

Kordzadeh, Azadeh | 2025

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 58165 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Ramazani Saadatabadi, Ahmad; Mashayekhan, Shohreh
  7. Abstract:
  8. In this study, the separation of melittin from honey bee venom using free-flow electrophoresis was investigated theoretically and experimentally. First, melittin, hyaluronidase, and phospholipase A2 were investigated using molecular dynamics and their properties were calculated. The results of molecular dynamics simulation were used for computational fluid dynamics simulation. The results of macroscopic simulation were used to optimize the chip dimensions and operating conditions. The optimal length, width, and depth of the chip were obtained as 50 mm, 20 mm, and 150 μm, respectively. Using the results of macroscopic simulation, the chip was fabricated using a wet etching method. Profilometry results showed that the channel surface had a uniform surface and an average roughness of 772 nm. Then, a melittin separation test was performed on the chip and the operating conditions were optimized. The optimal values for buffer flow rate, channel length, channel depth, electric field intensity and sample concentration are 50 µL/min, 50 mm, 150 µm, 1500 V/m and 1.5 mg/mL, respectively. The results of venom separation in the chip showed that phospholipase A2 was removed by 95% and melittin was obtained with 91% purity. This removed amount of phospholipase A2 causes the toxicity of honey bee venom to be eliminated and can be used for therapeutic applications. Next, carbon nanotubes functionalized with hydroxyl, amine and carboxyl groups were investigated as melittin carriers. The results of molecular dynamics simulation showed that the hydroxyl-functionalized carbon nanotube is a suitable carrier for melittin and did not change its structure and properties
  9. Keywords:
  10. Free Flow Electrophoresis ; Microchip ; Bee Venom ; Melittin ; Microfluidic Chip

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