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  1. Evaluating an extraction-free sample preparation method for multiplex detection of SARS-Cov-2, influenza A/B, and RSV with implementation on a microfluidic chip
Evaluating an extraction-free sample preparation method for multiplex detection of SARS-Cov-2, influenza A/B, and RSV with implementation on a microfluidic chip
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Evaluating an extraction-free sample preparation method for multiplex detection of SARS-Cov-2, influenza A/B, and RSV with implementation on a microfluidic chip

Ahmadi, F

Evaluating an extraction-free sample preparation method for multiplex detection of SARS-Cov-2, influenza A/B, and RSV with implementation on a microfluidic chip

Ahmadi, F ; Sharif University of Technology | 2024

339 Viewed
  1. Type of Document: Article
  2. DOI: 10.1016/j.diagmicrobio.2024.116325
  3. Publisher: 2024
  4. Abstract:
  5. Following the relaxation of COVID-19 restrictions, other respiratory viruses such as influenza and respiratory syncytial virus (RSV), whose transmission were decreased due to COVID-19 precautions, are rising again. Because of similar clinical features and reported co-infections, multiplex detection of SARS-CoV-2, influenza A/B, and RSV is required to use specific treatments. This study assessed an extraction-free sample preparation (heat treatment at 95°C for 3 minutes) for multiplex detection using rRT-PCR. Despite an observed Ct-delay (∆Ct) averageing 1.26 compared to the standard method, an acceptable total sensitivity of 92 % and a negative predictive value (NPV) of 96 % were obtained. Moreover, Implementation on a microfluidic chip demonstrated efficiency, maintaining an excellent correlation (R2=0.983) with the standard method. Combining this extraction-free procedure with rRT-PCR on a microfluidic chip seems promising, because it simplifies the design and reduces the cost and complexity of the integrated assay for multiplex detection of SARS-CoV-2, influenza A/B, and RSV. © 2024
  6. Keywords:
  7. Lab-on-a-chip ; Molecular diagnostics ; Point-of-care tests ; Real-time RT-PCR ; Respiratory infections ; Coinfection ; COVID-19 ; Humans ; Influenza, Human ; Lab-On-A-Chip Devices ; Respiratory Syncytial Virus Infections ; Respiratory Syncytial Viruses ; SARS-CoV-2 ; Controlled study ; Extraction ; Heat treatment ; Human respiratory syncytial virus ; Influenza A virus ; Influenza B virus ; Nonhuman ; Predictive value ; Real time polymerase chain reaction ; Sensitivity and specificity ; Severe acute respiratory syndrome coronavirus 2 ; Virus detection ; Coronavirus disease 2019 ; COVID-19 nucleic acid testing ; Devices ; Diagnosis ; Genetics ; Human ; Influenza ; Isolation and purification ; Lab on a chip ; Multiplex polymerase chain reaction ; Pneumovirus ; Procedures ; Respiratory syncytial virus infection ; Virology
  8. Source: Diagnostic Microbiology and Infectious Disease ; Volume 109, Issue 3 , 2024 ; 07328893 (ISSN)
  9. URL: https://pubmed.ncbi.nlm.nih.gov/38688146