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In-house device fabrication for multiple blood sampling of adult zebrafish, Danio rerio

Abdullah, N. R. B ; Sharif University of Technology | 2024

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  1. Type of Document: Article
  2. DOI: 10.1016/j.dwt.2024.100522
  3. Publisher: 2024
  4. Abstract:
  5. Multiple blood collection from an adult Danio rerio is important in most of metabolic studies. The method allows researcher to profile the metabolic dynamic from an individual fishes, which contribute to a high resolution of dataset. Currently, several devices have been developed for the purpose of blood withdrawal from Danio rerio. Limited accessibility to it hindering the log phase development of Danio rerio based studies. This study shows that the multiple blood collection from an adult Danio rerio can be achieved to the highest survival rate (94.75 %) using the in-house fabricated Z-fish needle. The fabricated device is cheaper and can be accessible in any developing Danio rerio laboratory. The device contains two main components: (i) the sampling syringe and (ii) the micro-capillary needle. Both components play its important role in contributing to the successful blood collection procedure. © 2024 The Author(s)
  6. Keywords:
  7. Danio rerio ; Micro-capillary needle ; Repeated blood collection ; Sampling syringe ; Adult ; Blood ; Cyprinid ; Metabolism ; Sampling ; Survival
  8. Source: Desalination and Water Treatment ; Volume 319 , 2024 ; 19443994 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S1944398624005563