Loading...

Synthesis and characterization of injectable thermosensitive hydrogel based on pluronic-grafted silk fibroin copolymer containing hydroxyapatite nanoparticles as potential for bone tissue engineering

Daneshvar, A ; Sharif University of Technology | 2024

59 Viewed
  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2024.134412
  3. Publisher: 2024
  4. Abstract:
  5. Injectable hydrogels are promising for bone tissue engineering due to their minimally invasive application and adaptability to irregular defects. This study presents the development of pluronic grafted silk fibroin (PF-127-g-SF), a temperature-sensitive graft copolymer synthesized from SF and modified PF-127 via a carbodiimide coupling reaction. The PF-127-g-SF copolymer exhibited a higher sol-gel transition temperature (34 °C at 16 % w/v) compared to PF-127 (23 °C), making it suitable for injectable applications. It also showed improved flexibility and strength, with a yielding point increase from <10 % to nearly 30 %. Unlike PF-127 gel, which degrades within 72 h in aqueous media, the PF-127-g-SF copolymer maintained a stable gel structure for over two weeks due to its robust crosslinked hydrogel network. Incorporating hydroxyapatite nanoparticles (n-HA) into the hydrogel reduced pore size and decreased swelling and degradation rates, extending structural stability to four weeks. Increasing n-HA concentration from 0 % to 20 % reduced porosity from 80 % to 66 %. Rheological studies indicated that n-HA enhanced the scaffold's strength and mechanical properties without altering gelation temperature. Cellular studies with MG-63 cells showed that n-HA concentration influenced cell viability and mineralization, highlighting the scaffold's potential in bone tissue engineering. © 2024 Elsevier B.V
  6. Keywords:
  7. Bone regeneration ; Nano-hydroxyapatite composite ; Biocompatible materials ; Bone and bones ; Durapatite ; Fibroins ; Injections ; Nanoparticles ; Rheology ; Biomechanics ; Cell engineering ; Degradation ; Gelation ; Nanocomposites ; Scaffolds (biology) ; Sol-gels ; Stability ; Alkaline phosphatase ; Cyanamide ; Hydrogel ; Lactate dehydrogenase ; Nanohydroxyapatite ; Poloxamer ; Reactive oxygen metabolite ; Biomaterial ; Hydroxyapatite ; Bone tissue engineering ; Hydroxyapatite composite ; Injectables ; PF-127 ; Pluronics ; Silk fibroin ; Thermo-sensitive hydrogel ; Apoptosis ; Apoptosis assay ; Bone tissue ; Cell viability ; Contact angle ; Cross linking ; Cytotoxicity ; Energy dispersive X ray spectroscopy ; Filtration ; Flow cytometry ; Freeze drying ; Genetic transfection ; Heat sensitivity ; Hydrogen bond ; Mineralization ; MTT assay ; Porosity ; Precipitation ; Proton nuclear magnetic resonance ; Scanning electron microscopy ; Shear flow ; Shear rate ; Solid state ; Swelling ; Thermogravimetry ; Tissue engineering ; Transmission electron microscopy ; Volatilization ; X ray diffraction ; Bone ; Chemistry ; Drug effect ; Flow kinetics ; Human ; Procedures ; Synthesis ; Temperature ; Tissue scaffold ; Pore size
  8. Source: International Journal of Biological Macromolecules ; Volume 277 , 2024 ; 01418130 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0141813024052176