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A novel approach of differentiation of adenoma and carcinoma in lung cancer based on biogenic in situ synthesis of gold nanostructures on various oligonucleotide motifs

Seifzadeh, S ; Sharif University of Technology | 2024

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  1. Type of Document: Article
  2. DOI: 10.1007/s00604-024-06744-z
  3. Publisher: 2024
  4. Abstract:
  5. A unique approach is introduced for constructing gold nanocrystals (AuNCs) with RNA motif–directed morphologies in a sequence-independent manner and its applications in the clinical area are described. By using this method, a label-free LSPR-based detection method for the SOX2OT transcript, long non-coding RNAs (lncRNAs), which is a prognostic indicator of poor survival in lung cancer patients is presented. For the first time, we examined how the structural changes of RNA after the heteroduplex formation with a specific DNA probe can change the morphology and LSPR band of AuNCs. Using this method, is was possible to differentiate lung squamous cell carcinoma from adenocarcinoma samples without a need for a prior amplification of the target lncRNA. The approach of using specific DNA probe enables the in situ synthesis of nanocrystals in a different way and expands this method for future translational medicine, particularly detection of specific RNA. Graphical Abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024
  6. Keywords:
  7. Biosensor ; Colorimetric detection ; Nanocrystals ; Adenocarcinoma ; Carcinoma, squamous cell ; DNA probes ; Gold ; Lung neoplasms ; Metal nanoparticles ; RNA, long noncoding ; Biological organs ; Nanoclay ; Oligonucleotides ; Long untranslated RNA ; Biogenics ; Gold nanocrystals ; Gold nanostructures ; In-situ synthesis ; ITS applications ; Label free ; RNA motifs ; Chemistry ; Diagnosis ; Genetics ; Lung tumor ; Squamous cell carcinoma ; Lung cancer
  8. Source: Microchimica Acta ; Volume 191, Issue 11 , 2024 ; 00263672 (ISSN)
  9. URL: https://link.springer.com/article/10.1007/s00604-024-06744-z