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Antibacterial activity of a silver-incorporated vancomycin-modified mesoporous silica against methicillin-resistant staphylococcus aureus

Chamani, M ; Sharif University of Technology | 2024

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  1. Type of Document: Article
  2. DOI: 10.1177/08853282241274517
  3. Publisher: 2024
  4. Abstract:
  5. Since conventional antibiotics are almost ineffective on methicillin-resistant Staphylococcus aureus (MRSA) strains, designing their antibacterial alternatives is necessary. Besides, the use of vancomycin is applied for specific detection of the bacteria. Silver-incorporated vancomycin-modified mesoporous silica nanoparticles (MSNs@Van@Ag NPs) were designed for detection and treatment of MRSA bacteria. Mesoporous silica nanoparticles (MSNs) were synthesized through the template method, modified with vancomycin, and finally incorporated with silver nanoparticles (Ag NPs). The MSNs@Van@Ag NPs with a homogenously spherical shape, average size of 50-100 nm, surface area of 955.8 m2/g, and thermal stability up to 200°C were successfully characterized. The amount of Ag incorporated into the MSNs@Van@Ag was calculated at 3.9 ppm and the release amount of Ag was received at 2.92 ppm (75%) after 100 h. The in vitro antibacterial susceptibility test showed the MIC of 100 μg mL−1 for MSNs@Van and 50 μg mL−1 for MSNs@Van@Ag, showing in vitro enhanced effect of Ag and vancomycin in the bactericidal process. An in vivo acute pneumonia model was performed and biochemical assays and pathological studies confirmed the nanomedicine’s short-term safety for in vivo application. Cytokine assay using ELISA showed that MSN@Van@Ag causes a reduction of pro-inflammatory cytokines and bacterial proliferation leading to alleviation of inflammatory response. © The Author(s) 2024
  6. Keywords:
  7. Silver ; Anti-bacterial agents ; Mice ; Microbial sensitivity tests ; Porosity ; Mesoporous materials ; Nanoclay ; Silica nanoparticles ; Staphylococcus aureus ; Antibiotic agent ; Interleukin 6 ; Ketamine ; Mesoporous silica nanoparticle ; Silver nanoparticle ; Tumor necrosis factor ; Xylazine ; Antiinfective agent ; Metal nanoparticle ; Silicon dioxide ; Antibacterials ; Aureus strain ; In-vitro ; In-vivo ; Mesoporous silicas ; Modified mesoporous silicas ; Vancomycin ; Acute kidney tubule necrosis ; Animal experiment ; Animal model ; Animal tissue ; Antibacterial activity ; Antibiotic sensitivity ; Coagulative necrosis ; Colony forming unit ; Controlled study ; Dialysis ; Drug synthesis ; Enzyme linked immunosorbent assay ; Fourier transform infrared spectroscopy ; Fourier transform mass spectrometry ; High performance liquid chromatography ; Hyperemia ; Immunoadsorption ; Inductively coupled plasma mass spectrometry ; Inflammation ; Light microscopy ; Methicillin resistant Staphylococcus aureus ; Minimum inhibitory concentration ; Minimum lethal dose ; Nonhuman ; Optical density ; pH ; Pneumonia ; Rat ; Thermogravimetry ; Transmission electron microscopy ; X ray diffraction ; Chemistry ; Drug effect ; Drug therapy ; Mouse ; Staphylococcus infection ; Mesopores
  8. Source: Journal of Biomaterials Applications ; Volume 39, Issue 5 , 2024 , Pages 439-454 ; 08853282 (ISSN)
  9. URL: https://journals.sagepub.com/doi/10.1177/08853282241274517