Generation of Cancer Spheroids by Microfluidic Droplet Method for Tests

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Naseri, Tahoora | 2024

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 57700 (08)
University: Sharif University of Technology
Department: Mechanical Engineering
Advisor(s): Shamloo, Amir; Siamak Kazemzadeh Hannani
Abstract:
Cancer spheroids are complex three-dimensional models of cancer cells that closely resemble in vivo tumors. Studying these models is essential for better understanding tumor biology, developing new drugs, and testing anti-cancer therapies. In this paper, we present a novel method for generating cancer spheroids using droplet-based microfluidics. This method is based on the formation of small droplets containing cancer cells in a microfluidic channel, which leads to the self-assembly of spheroids. We employ metal-organic framework (MOF) nanoparticles as drug carriers to enhance drug delivery to spheroids. ZIF-8, a porous MOF with the ability to load and release drugs in a controlled manner, was selected as the drug carrier nanoparticle. Our results demonstrate that spheroids generated using this method exhibit similar structure and function to in vivo tumors. Moreover, the use of ZIF-8 as a drug carrier significantly increases the drug delivery efficiency to spheroids. This novel method for generating cancer spheroids using droplet-based microfluidics and MOF nanoparticles provides a powerful tool for studying and treating cancer
Keywords:
Droplet-Based Microfluidics ; Metal-Organic Framework ; Drug Delivery ; Zeolite Imidazolate Framework ; Cancer Spheroids

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