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Investigating the Effect of Amino Linker Exchange in the ZIF-8 Metal-Organic Framework on the Loading and Release of 5-FU Drug

Mirzanejad, Sahba | 2024

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 57444 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Bagherzadeh, Mojtaba
  7. Abstract:
  8. In this study, the effect of amino linker exchange in ZIF-8 metal-organic frameworks on the loading and release of 5-Fluorouracil was investigated. For this purpose, at first ZIF-8 nanoparticles were synthesized. Then, the Solvent-assisted linker exchange method was utilized to exchange part of 2-methylimidazole linkers with 3-amino-1,2,4-triazole in ZIF-8 structure. Three amine-functionalized ZIF-8 with 22%, 53%, and 74% of Atz exchange were synthesized. The drug was loaded into ZIF-8, ZIF-8A(22%), ZIF-8A(53%) ,and ZIF-8A(74%). The prepared nanocarriers were characterized by Nuclear Magnetic Resonance Spectroscopy (NMR), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction Spectroscopy (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), UV-Vis spectroscopy, and Zeta potential analysis. Furthermore, drug encapsulation efficiency results showed 12% for 5-FU@ZIF-8 which increased to 48% for 5-FU@ZIF-8A(53%). This increase in drug encapsulation efficiency is due to the presence of the amine functional group in the structure, which improves the host-guest interactions between drug molecules and linkers. Also, the results of in vitro experiments exhibited the pH-responsive behavior of nanocarriers and slower release for 5-FU@ZIF-8A(53%) compared to 5-FU@ZIF-8. Moreover, MTT assay was performed on MCF-7 and HFF-2 cell lines. The results obtained from MTT assay demonstrate less cytotoxicity for 5-FU@ZIF-8A(53%) compared to 5-FU@ZIF-8 after 24 h treatment
  9. Keywords:
  10. Metal-Organic Framework ; Drug Delivery ; Zeolite Imidazolate Framework ; Solvent-Assisted Linker Exchange ; 5-Fluorouracil ; 2-Methyl Imidazole

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