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Partitioning of penicillin G acylase in aqueous two-phase systems of poly(ethylene glycol) 20000 or 35000 and potassium dihydrogen phosphate or sodium citrate

Pazuki, G ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1021/je900319s
  3. Abstract:
  4. The partitioning of penicillin G acylase in aqueous two-phase systems (ATPS's) containing poly(ethylene glycol) (PEG) 20000 or 35000 and potassium dihydrogen phosphate (KH2PO4) or sodium citrate (C 6H5Na3O7 ·5H2O) has been measured at three temperatures, (301.2, 307.2, and 310.2) K. The effects of temperature, polymer molecular weight, and polymer and salt concentrations on the partitioning of penicillin G in the ATPS were studied. The experimental data showed that the composition of salt has a large effect on partitioning of penicillin G in ATPS, and the temperature of the system has a small effect on the partitioning. The UNIFAC-FV group contribution model (Pazuki et al., Ind. Eng. Chem. Res. 2009, 48, 4109-4118) was used in correlating the partition coefficients of penicillin G in polymer + ATPS
  5. Keywords:
  6. Aqueous two phase system ; Effects of temperature ; Experimental data ; Group contribution model ; Partition coefficient ; Penicillin G ; Penicillin G acylase ; Polymer molecular weight ; Potassium dihydrogen phosphate ; Salt concentration ; Sodium citrate ; Three temperature ; UNIFAC-FV ; Ethylene ; Ethylene glycol ; Polyethylene glycols ; Polyethylene oxides ; Drug products
  7. Source: Journal of Chemical and Engineering Data ; Volume 55, Issue 1 , 2010 , Pages 243-248 ; 00219568 (ISSN)
  8. URL: http://pubs.acs.org/doi/abs/10.1021/je900319s?src=recsys